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Alpha Synuclein Polyclonal antibody

Alpha Synuclein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 10842-1-AP

Print datasheet

Synonyms

a-synuclein, SNCA, Alpha-synuclein, PARK1, Non-A4 component of amyloid precursor



经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy Alpha Synuclein Ready-To-Use IHC Kit. Alpha Synuclein primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10842-1-AP targets Alpha Synuclein in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, yeast, zebra finch
Immunogen Alpha Synuclein fusion protein Ag1285 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synuclein, alpha (non A4 component of amyloid precursor)
Synonyms a-synuclein, SNCA, Alpha-synuclein, PARK1, Non-A4 component of amyloid precursor
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 15-19 kDa
GenBank Accession NumberBC013293
Gene Symbol Alpha Synuclein
Gene ID (NCBI) 6622
RRIDAB_2192672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP37840
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. Α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

实验方案

Product Specific Protocols
WB protocol for Alpha Synuclein antibody 10842-1-APDownload protocol
IHC protocol for Alpha Synuclein antibody 10842-1-APDownload protocol
IF protocol for Alpha Synuclein antibody 10842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Transl Neurodegener

Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models

Authors - Alice Drobny
mouseIF

Aging Dis

MSC-Derived Exosomes can Enhance the Angiogenesis of Human Brain MECs and Show Therapeutic Potential in a Mouse Model of Parkinson's Disease.

Authors - Chunling Xue
humanIHC

Acta Neuropathol Commun

Mixed pathologies in pancreatic β cells from subjects with neurodegenerative diseases and their interaction with prion protein.

Authors - Ivan Martinez-Valbuena
mouseWB,IHC

Aging Cell

Poly (ADP-ribose) polymerase 1 inhibition prevents neurodegeneration and promotes α-synuclein degradation via transcription factor EB-dependent autophagy in mutant α-synucleinA53T model of Parkinson's disease.

Authors - Kanmin Mao
humanWB

Acta Pharmacol Sin

Roflupram protects against rotenone-induced neurotoxicity and facilitates α-synuclein degradation in Parkinson's disease models.

Authors - Wen-Li Dong
mouseWB, IHC, IF

J Neurosci

Mutant alpha-synuclein causes age-dependent neuropathology in monkey brain.

Authors - Weili Yang