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SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10269-1-AP

Print datasheet

Synonyms

SOD, IPOA, hSod1, homodimer, EC:1.15.1.1

验证数据展示

  • WB analysis of HEK-293 using 10269-1-AP

    WB analysis of HEK-293 using 10269-1-AP

    WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.

  • WB analysis using 10269-1-AP

    WB analysis using 10269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 10269-1-AP

    IHC staining of human liver cancer using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOD1 antibody included
  • IHC staining of human liver cancer using 10269-1-AP

    IHC staining of human liver cancer using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOD1 antibody included
  • IF Staining of HEK-293 using 10269-1-AP

    IF Staining of HEK-293 using 10269-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SOD1 antibody (10269-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HEK-293 using 10269-1-AP

    IP experiment of HEK-293 using 10269-1-AP

    IP result of anti-SOD1 (IP:10269-1-AP, 4ug; Detection:10269-1-AP 1:6000) with HEK-293 cells lysate 1470 ug.

  • IHC staining of human colon using 10269-1-AP

    IHC staining of human colon using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOD1 antibody included
  • IF Staining of mouse brain using 10269-1-AP

    IF Staining of mouse brain using 10269-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOD1 antibody (10269-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10269-1-AP targets SOD1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, bovine, goat
Immunogen SOD1 fusion protein Ag0335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name superoxide dismutase 1, soluble
Synonyms SOD, IPOA, hSod1, homodimer, EC:1.15.1.1
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC001034
Gene Symbol SOD1
Gene ID (NCBI) 6647
RRIDAB_2193750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00441
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

实验方案

Product Specific Protocols
WB protocol for SOD1 antibody 10269-1-APDownload protocol
IHC protocol for SOD1 antibody 10269-1-APDownload protocol
IF protocol for SOD1 antibody 10269-1-APDownload protocol
IP protocol for SOD1 antibody 10269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons

Authors - Zaida Álvarez
View Article
mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
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mouseWB

Bioact Mater

Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration

Authors - Hao Wu
View Article
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article
humanWB

Nat Commun

Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.

Authors - Yun Liao
View Article
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang
View Article