验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse brain tissue, THP-1 cells, SKOV-3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SOS1 Ready-To-Use IHC Kit. SOS1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
55041-1-AP targets SOS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | son of sevenless homolog 1 (Drosophila) |
| 别名 | GF1, GGF1, GINGF, NS4, Son of sevenless homolog 1, SOS 1, SOS1 |
| 计算分子量 | 152 kDa |
| 观测分子量 | 152 kDa |
| GenBank蛋白编号 | NM_005633 |
| 基因名称 | SOS1 |
| Gene ID (NCBI) | 6654 |
| RRID | AB_10804659 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q07889 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOS1 antibody 55041-1-AP | Download protocol |
| IHC protocol for SOS1 antibody 55041-1-AP | Download protocol |
| IF protocol for SOS1 antibody 55041-1-AP | Download protocol |
| IP protocol for SOS1 antibody 55041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cells Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer. | ||
Pathol Res Pract KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway | ||
Cancers (Basel) Lactate Oxidase Disrupts Lactate-Activated RAS and PI3K Oncogenic Signaling | ||
Adv Sci (Weinh) Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer | ||
Sci Adv HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis |
