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  • Featured Product
  • KD/KO Validated

SOX2 Monoclonal antibody, PBS Only

SOX2 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B9B10

Cat No : 66411-1-PBS

Print datasheet

Synonyms

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

验证数据展示

  • WB analysis of HEK-293 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB analysis of HEK-293 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB result of SOX2 antibody (66411-1-Ig, 1:4000) with si-Control and si-SOX2 transfected HEK293 cells.. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • WB analysis using 66411-1-Ig (same clone as 66411-1-PBS)

    WB analysis using 66411-1-Ig (same clone as 66411-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

    IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOX2 antibody included
  • IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOX2 antibody included
  • WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB result of SOX2 antibody (66411-1-Ig; 1:3000; room temperature for 1.5 hours) with negative control and SOX2 knockout U-251 cells. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    FC experiment of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    1X10^6 U-251 cells were intracellularly stained with 0.5 ug Anti-Human SOX2 (66411-1-Ig, Clone:4B9B10) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    IF Staining of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using 66411-1-Ig (SOX2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SOX2 Ready-To-Use IHC Kit. SOX2 primary antibody included.

产品信息

66411-1-PBS targets SOX2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications WB, IF, FC, IHC, ELISA Application Description
Tested Reactivity human, pig, mouse, rat
Immunogen SOX2 fusion protein Ag13635 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 2
Synonyms ANOP3, MCOPS3, SOX2, Transcription factor SOX 2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC013923
Gene Symbol SOX2
Gene ID (NCBI) 6657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48431
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2.