验证数据展示
经过测试的应用
| Positive WB detected in | U-251 cells, Jurkat cells, C6 cells |
| Positive IHC detected in | rat testis tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14010-1-AP targets SOX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SOX6 fusion protein Ag5111 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 6 |
| 别名 | HSSOX6, SOX6, SOXD, Transcription factor SOX 6 |
| 计算分子量 | 92 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC047064 |
| 基因名称 | SOX6 |
| Gene ID (NCBI) | 55553 |
| RRID | AB_2196095 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35712 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Sex-determining Region Y (SRY)-related high-mobilitygroup box 6 (SOX6) is a transcription factor that was discovered in 1990s while searching for Sry-related genes. SOX6 belongs to the subfamily D of SOX protein and can recognize the conserved sequence of "(A/T)(A/T)CAA(A/ T)G" within the transcriptional regulation region of the target gene with its HMG domain. Sox6 contains DNA binding domain and binds to the minor groove of DNA. The ability of SOX6 to interact with different proteins at different places and times, makes it an intricate protein with roles in multiple processes. SOX6 has been implicated in diabetic nephropathy, adipogenesis, cardiomyopathy, inflammatory bowel disease, and cancer. (PMID: 33230925)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX6 antibody 14010-1-AP | Download protocol |
| IHC protocol for SOX6 antibody 14010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination. | ||
Int J Mol Med miRNA‑342 suppresses renal interstitial fibrosis in diabetic nephropathy by targeting SOX6. | ||
Nephrology (Carlton) CircHOMER1 aggravates oxidative stress, inflammation and ECM deposition in high glucose-induced human mesangial cells | ||
bioRxiv Eed controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest | ||
Matrix Biol SERPINA3 is a marker of cartilage differentiation and is essential for the expression of extracellular matrix genes during early chondrogenesis |
