SOX6 Polyclonal antibody

SOX6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14010-1-AP

Print datasheet

Synonyms

HSSOX6, SOX6, SOXD, Transcription factor SOX 6



经过测试的应用

Positive WB detected inU-251 cells, Jurkat cells, C6 cells
Positive IHC detected inrat testis tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SOX6 Ready-To-Use IHC Kit. SOX6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14010-1-AP targets SOX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Immunogen SOX6 fusion protein Ag5111 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 6
Synonyms HSSOX6, SOX6, SOXD, Transcription factor SOX 6
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC047064
Gene Symbol SOX6
Gene ID (NCBI) 55553
RRIDAB_2196095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35712
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Sex-determining Region Y (SRY)-related high-mobilitygroup box 6 (SOX6) is a transcription factor that was discovered in 1990s while searching for Sry-related genes. SOX6 belongs to the subfamily D of SOX protein and can recognize the conserved sequence of "(A/T)(A/T)CAA(A/ T)G" within the transcriptional regulation region of the target gene with its HMG domain. Sox6 contains DNA binding domain and binds to the minor groove of DNA. The ability of SOX6 to interact with different proteins at different places and times, makes it an intricate protein with roles in multiple processes. SOX6 has been implicated in diabetic nephropathy, adipogenesis, cardiomyopathy, inflammatory bowel disease, and cancer. (PMID: 33230925)

实验方案

Product Specific Protocols
WB protocol for SOX6 antibody 14010-1-APDownload protocol
IHC protocol for SOX6 antibody 14010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
mouseWB

Nat Commun

VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.

Authors - Yuki Miyamoto
mouseWB

Int J Mol Med

miRNA‑342 suppresses renal interstitial fibrosis in diabetic nephropathy by targeting SOX6.

Authors - Zhen-Huan Jiang
humanWB

Nephrology (Carlton)

CircHOMER1 aggravates oxidative stress, inflammation and ECM deposition in high glucose-induced human mesangial cells

Authors - Shi Shu
humanWB

Biochem Biophys Res Commun

MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway.

Authors - Wang Li
mouseWB,IF

Cell Prolif

LncRNA Riken-201 and Riken-203 modulates neural development by regulating the Sox6 through sequestering miRNAs.

Authors - Lei Zhang