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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)

    WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included
  • IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included
  • IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included
  • IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included

SPI1 Monoclonal antibody, PBS Only

SPI1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66618-2-PBS
CloneNo. 2H3D3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

SFPI1, PU.1, OF, 31 kDa-transforming protein, 31 kDa transforming protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SPI1 Ready-To-Use IHC Kit. SPI1 primary antibody included.

产品信息

66618-2-PBS targets SPI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name spleen focus forming virus (SFFV) proviral integration oncogene spi1
Synonyms SFPI1, PU.1, OF, 31 kDa-transforming protein, 31 kDa transforming protein
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberNM_003120
Gene Symbol SPI1
Gene ID (NCBI) 6688
ENSEMBL Gene IDENSG00000066336
RRIDAB_2881978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17947
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.