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  • Featured Product
  • KD/KO Validated

SREBF1 Polyclonal antibody

SREBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 14088-1-AP

Print datasheet

Synonyms

SREBP 1c, SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1

验证数据展示

  • WB analysis using 14088-1-AP

    WB analysis using 14088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 14088-1-AP

    IP experiment of L02 using 14088-1-AP

    IP result of anti-SREBF1 (IP:14088-1-AP, 4ug; Detection:14088-1-AP 1:600) with L02 cells lysate 1500ug.

  • IHC staining of human kidney using 14088-1-AP

    IHC staining of human kidney using 14088-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SREBF1 antibody included
  • IHC staining of human kidney using 14088-1-AP

    IHC staining of human kidney using 14088-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SREBF1 antibody included
  • IF Staining of HeLa using 14088-1-AP

    IF Staining of HeLa using 14088-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14088-1-AP (SREBF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HeLa using 14088-1-AP

    WB analysis of HeLa using 14088-1-AP

    WB result of SREBF1 antibody (14088-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells.

  • WB analysis of HeLa using 14088-1-AP

    WB analysis of HeLa using 14088-1-AP

    WB result of SREBF1 antibody (14088-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells.

  • WB analysis using 14088-1-AP

    WB analysis using 14088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14088-1-AP

    WB analysis using 14088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SREBF1 Ready-To-Use IHC Kit. SREBF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14088-1-AP targets SREBF1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, bovine, goat
Immunogen SREBF1 fusion protein Ag5219 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterol regulatory element binding transcription factor 1
Synonyms SREBP 1c, SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1
Calculated Molecular Weight 1177 aa, 125 kDa
Observed Molecular Weight 125 kDa, 68 kDa
GenBank Accession NumberBC063281
Gene Symbol SREBF1
Gene ID (NCBI) 6720
RRIDAB_2255217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.

实验方案

Product Specific Protocols
WB protocol for SREBF1 antibody 14088-1-APDownload protocol
IHC protocol for SREBF1 antibody 14088-1-APDownload protocol
IF protocol for SREBF1 antibody 14088-1-APDownload protocol
IP protocol for SREBF1 antibody 14088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A metastasis map of human cancer cell lines.

Authors - Xin Jin
  • KO Validated
View Article
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
mouseIHC

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
View Article
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
  • KD Validated
View Article
mouseWB

Nat Commun

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

Authors - Jinjie Duan
View Article
human

Hepatology

PMID: 29729186

Authors - Huixia Zhang
View Article