验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A375 cells, mouse pancreas tissue, rat pancreas tissue, Caco-2 cells |
| Positive IHC detected in | human stomach cancer tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12212-1-AP targets SSBP1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 | SSBP1 fusion protein Ag2860 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | single-stranded DNA binding protein 1 |
| 别名 | Single-stranded DNA-binding protein, mitochondrial, PWP1-interacting protein 17, PWP1 interacting protein 17, Mt-SSB, MtSSB |
| 计算分子量 | 148 aa, 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC000895 |
| 基因名称 | SSBP1 |
| Gene ID (NCBI) | 6742 |
| RRID | AB_2195320 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q04837 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SSBP1 antibody 12212-1-AP | Download protocol |
| IHC protocol for SSBP1 antibody 12212-1-AP | Download protocol |
| IF protocol for SSBP1 antibody 12212-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. | ||
Mol Cell Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion. | ||
Sci Adv The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
| ||
Nucleic Acids Res Top3α is the replicative topoisomerase in mitochondrial DNA replication. |
