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验证数据展示

  • WB analysis of HEK-293 using 26581-1-AP

    WB analysis of HEK-293 using 26581-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26581-1-AP (ST13 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 26581-1-AP

    IP experiment of HEK-293 using 26581-1-AP

    IP result of anti-ST13 (IP:26581-1-AP, 4ug; Detection:26581-1-AP 1:2000) with HEK-293 cells lysate 1800ug.

  • IHC staining of human colon cancer using 26581-1-AP

    IHC staining of human colon cancer using 26581-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ST13 antibody included
  • IHC staining of human colon cancer using 26581-1-AP

    IHC staining of human colon cancer using 26581-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ST13 antibody included

ST13 Polyclonal antibody

ST13 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26581-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

Progesterone receptor-associated p48 protein, HSPABP1, HSPABP, Hsc70-interacting protein, Aging-associated protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ST13 Ready-To-Use IHC Kit. ST13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26581-1-AP targets ST13 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 ST13 fusion protein Ag24779 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
别名 Progesterone receptor-associated p48 protein, HSPABP1, HSPABP, Hsc70-interacting protein, Aging-associated protein 2
观测分子量 45-50 kDa
GenBank蛋白编号BC139724
基因名称 ST13
Gene ID (NCBI) 6767
RRIDAB_2880560
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50502
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ST13 antibody 26581-1-APDownload protocol
IHC protocol for ST13 antibody 26581-1-APDownload protocol
IP protocol for ST13 antibody 26581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
View Article
mouseWB

Cancer Res

N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma

Authors - Duygu Kuzuoglu-Ozturk
View Article