STAT2 Polyclonal antibody

STAT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 51075-2-AP

Print datasheet

Synonyms

ISGF 3, P113, STAT113, STAT2



经过测试的应用

Positive WB detected inMCF7 cells, multi-cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STAT2 Ready-To-Use IHC Kit. STAT2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

51075-2-AP targets STAT2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited Applications WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 2, 113kDa
Synonyms ISGF 3, P113, STAT113, STAT2
Calculated Molecular Weight 851 aa, 98 kDa
Observed Molecular Weight 100-113 kDa
GenBank Accession NumberBC051284
Gene Symbol STAT2
Gene ID (NCBI) 6773
RRIDAB_1167479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52630
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human STAT2. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

实验方案

Product Specific Protocols
WB protocol for STAT2 antibody 51075-2-APDownload protocol
IHC protocol for STAT2 antibody 51075-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

J Clin Invest

Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy.

Authors - Yan-Ping Xu
humanWB

BMC Vet Res

Curcumol inhibits EMCV replication by activating CH25H and inhibiting the formation of ROs

Authors - Jiangang Zheng
humanWB

J Transl Med

Pretreatment of UC-MSCs with IFN-α2 improves treatment of liver fibrosis by recruiting neutrophils

Authors - Ye Xie
humanWB

Arthritis Res Ther

Potential role of RhoA GTPase regulation in type interferon signaling in systemic lupus erythematosus

Authors - Wei Fan
mouseWB

Viruses

Interferon-Stimulated Gene 15 Knockout in Mice Impairs IFNα-Mediated Antiviral Activity

Authors - Chen Li