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- KD/KO Validated
STAT5A/B Polyclonal antibody
STAT5A/B Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, 3T3-L1 cells, Daudi cells, DU 145 cells, SGC-7901 cells |
Positive IP detected in | K-562 cells, 3T3-L1 cells |
Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13179-1-AP targets STAT5A/B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine, cow |
Immunogen | STAT5A/B fusion protein Ag3846 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | signal transducer and activator of transcription 5A |
Synonyms | STAT5A, STAT5, MGF |
Calculated Molecular Weight | 794 aa, 92 kDa |
Observed Molecular Weight | 90-95 kDa |
GenBank Accession Number | BC027036 |
Gene Symbol | STAT5A |
Gene ID (NCBI) | 6776 |
RRID | AB_2196760 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P42229 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for STAT5A/B antibody 13179-1-AP | Download protocol |
IHC protocol for STAT5A/B antibody 13179-1-AP | Download protocol |
IP protocol for STAT5A/B antibody 13179-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Chemosphere MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.
| ||
Endocrinology A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration | ||
Front Oncol In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer | ||
Ecotoxicol Environ Saf Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells. | ||
J Cell Mol Med IL-27 Rα+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation. | ||
Cancer Cell Int LNK promotes the growth and metastasis of triple negative breast cancer via activating JAK/STAT3 and ERK1/2 pathway. |