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  • KD/KO Validated

STAT5A/B Polyclonal antibody

STAT5A/B Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13179-1-AP

Print datasheet

Synonyms

STAT5A, STAT5, MGF



经过测试的应用

Positive WB detected inK-562 cells, 3T3-L1 cells, Daudi cells, DU 145 cells, SGC-7901 cells
Positive IP detected inK-562 cells, 3T3-L1 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13179-1-AP targets STAT5A/B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, cow
Immunogen STAT5A/B fusion protein Ag3846 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 5A
Synonyms STAT5A, STAT5, MGF
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2196760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against 445-794 aa of human STAT5A, the homology between this segment and the corresponding segment of human STAT5B is 92%. It shows that this antibody can cross-react with STAT5B.

实验方案

Product Specific Protocols
WB protocol for STAT5A/B antibody 13179-1-APDownload protocol
IHC protocol for STAT5A/B antibody 13179-1-APDownload protocol
IP protocol for STAT5A/B antibody 13179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

Chemosphere

MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.

Authors - Yuezhu Zhang
  • KD Validated
humanWB

Endocrinology

A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration

Authors - Ruonan Li
humanIHC,WB

Front Oncol

In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer

Authors - Xiaodi Gong
ratWB

Ecotoxicol Environ Saf

Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.

Authors - Yuezhu Zhang
mouseWB

J Cell Mol Med

IL-27 Rα+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation.

Authors - Shanshan Zhao
humanWB

Cancer Cell Int

LNK promotes the growth and metastasis of triple negative breast cancer via activating JAK/STAT3 and ERK1/2 pathway.

Authors - Jianxin Lv