STAT5A Monoclonal antibody

STAT5A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F10F6

Cat no : 66459-1-Ig

Print datasheet

Synonyms

MGF, STAT5, STAT5A



经过测试的应用

Positive WB detected inRAW 264.7 cells, U-937 cells, rat spleen tissue, pig spleen tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66459-1-Ig targets STAT5A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman
Immunogen STAT5A fusion protein Ag19546 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name signal transducer and activator of transcription 5A
Synonyms MGF, STAT5, STAT5A
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2881828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

实验方案

Product Specific Protocols
WB protocol for STAT5A antibody 66459-1-IgDownload protocol
IF protocol for STAT5A antibody 66459-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

Intrinsic RIG-I restrains STAT5 activation to modulate anti-tumor activity of CD8+ T cells

Authors - Xinyi Jiang