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  • Featured Product
  • KD/KO Validated

SYK Monoclonal antibody, PBS Only

SYK Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4C4A12

Cat no : 66721-1-PBS

Print datasheet

Synonyms

Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82, EC:2.7.10.2

验证数据展示

  • WB analysis using 66721-1-Ig (same clone as 66721-1-PBS)

    WB analysis using 66721-1-Ig (same clone as 66721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66721-1-Ig (SYK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

    IF Staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66721-1-Ig (SYK antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 66721-1-Ig (same clone as 66721-1-PBS)

    WB analysis of Raji using 66721-1-Ig (same clone as 66721-1-PBS)

    WB result of SYK antibody (66721-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 66721-1-Ig (same clone as 66721-1-PBS)

    WB analysis of THP-1 using 66721-1-Ig (same clone as 66721-1-PBS)

    THP-1 (WT and SYK KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 66721-1-Ig incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

  • IP experiment of MDA-MB-231 using 66721-1-Ig (same clone as 66721-1-PBS)

    IP experiment of MDA-MB-231 using 66721-1-Ig (same clone as 66721-1-PBS)

    MDA-MB-231 lysates prepared and IP of SYK performed using 2.0 μg of 66721-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

    IHC staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66721-1-Ig (SYK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

产品信息

66721-1-PBS targets SYK in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Tested Reactivity human
Immunogen SYK fusion protein Ag17654 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name spleen tyrosine kinase
Synonyms Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82, EC:2.7.10.2
Calculated Molecular Weight 635 aa, 72 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
RRIDAB_2882072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43405
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)