• Featured Product
  • KD/KO验证

TAP1 Polyclonal antibody

TAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

RING4, PSF-1, PSF1, EC:7.4.2.14, ATP-binding cassette sub-family B member 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHCT 116 cells, HepG2 cells, mouse skeletal muscle tissue, HeLa cells, mouse spleen tissue, SW480 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TAP1 Ready-To-Use IHC Kit. TAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11114-1-AP targets TAP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, RIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 TAP1 fusion protein Ag1619 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
别名 RING4, PSF-1, PSF1, EC:7.4.2.14, ATP-binding cassette sub-family B member 2
计算分子量 81 kDa
观测分子量 70-81 kDa
GenBank蛋白编号BC014081
基因名称 TAP1
Gene ID (NCBI) 6890
RRIDAB_2200201
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03518
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

实验方案

Product Specific Protocols
WB protocol for TAP1 antibody 11114-1-APDownload protocol
IHC protocol for TAP1 antibody 11114-1-APDownload protocol
IF protocol for TAP1 antibody 11114-1-APDownload protocol
IP protocol for TAP1 antibody 11114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gastroenterology

microRNA 125a Regulates MHC-I Expression on Esophageal Adenocarcinoma Cells, Associated With Suppression of Antitumor Immune Response and Poor Outcomes of Patients.

Authors - Luigi Mari
humanIHC

Nat Commun

Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes.

Authors - Karama Asleh
humanWB,IP

Proc Natl Acad Sci U S A

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska
mouseWB

Theranostics

Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.

Authors - Jingyao Tu
humanIHC

Int J Mol Sci

Antigen Peptide Transporter 1 (TAP1) Promotes Resistance to MEK Inhibitors in Pancreatic Cancers.

Authors - Boya Li
humanWB

J Immunol

Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.

Authors - Zhangchuan Xia
  • KD Validated
Loading...