• Featured Product
  • KD/KO Validated

TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12892-1-AP

Print datasheet

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43, TDP-43 (C-terminal)



经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, HeLa cells, C6 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, Neuro-2a cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12892-1-AP targets TDP-43 (C-terminal) in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications chIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, zebrafish, Drosophila
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43, TDP-43 (C-terminal)
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43-45 kDa, 35 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
RRIDAB_2200505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

实验方案

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IF protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IP protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.

Authors - Anna-Leigh Brown
humanWB,IHC

Science

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.

Authors - Haiyang Yu
  • KO Validated
human,mouseIF

Nature

TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle.

Authors - Thomas O Vogler
mouseWB,IF

Nature

Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.

Authors - Lindsay A Becker
humanIF

Nat Med

Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis.

Authors - Payam Mohassel
humanWB

Nat Cell Biol

Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition

Authors - Shan Lu