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TDP-43 (C-terminal) Polyclonal antibody
TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA and More (4)
Conjugate
Unconjugated
238
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, mouse brain tissue, HeLa cells, C6 cells |
Positive IP detected in | HeLa cells, mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-Fro detected in | mouse brain tissue |
Positive IF/ICC detected in | HeLa cells, Neuro-2a cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:2000-1:8000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12892-1-AP targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, chIP, RIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, chicken, zebrafish, drosophila |
Immunogen | fusion protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | TAR DNA binding protein |
Synonyms | TDP 43, TARDBP, TDP43, TDP-43, TAR DNA-binding protein 43 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 43-45 kDa, 35 kDa |
GenBank Accession Number | BC001487 |
Gene Symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
RRID | AB_2200505 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13148 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
实验方案
Product Specific Protocols | |
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WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.
| ||
Nature Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. | ||
Nat Cell Biol Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition |