验证数据展示

IHC staining of mouse brain using 12892-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TDP-43 antibody included
IF Staining of HeLa using 12892-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB analysis of A549 cells using 12892-1-AP
A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.
WB analysis of HeLa using 12892-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IF Staining of mouse brain using 12892-1-AP
Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IP experiment of HeLa using 12892-1-AP
IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.
FC experiment of HeLa using 12892-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (12892-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
IHC staining of rat brain using 12892-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TDP-43 antibody included
IF Staining of mouse brain using 12892-1-AP
Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

Cat No. 12892-1-AP

产品说明书

引用文献(247)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA and More (4)

别名

TDP 43, TARDBP, TDP43, TDP-43, TAR DNA-binding protein 43

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

Biotin CoraLite® Plus 488 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (247)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, mouse brain tissue, HeLa cells, C6 cells
Positive IP detected in	HeLa cells, mouse brain tissue
Positive IHC detected in	rat brain tissue, human gliomas tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	HeLa cells, Neuro-2a cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 13 publications below
WB	See 149 publications below
IHC	See 50 publications below
IF	See 92 publications below
IP	See 10 publications below
CoIP	See 2 publications below
ChIP	See 2 publications below
RIP	See 1 publications below

产品信息

12892-1-AP targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, chIP, RIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, monkey, chicken, zebrafish, drosophila
免疫原	fusion protein 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	TAR DNA binding protein
别名	TDP 43, TARDBP, TDP43, TDP-43, TAR DNA-binding protein 43
计算分子量	43 kDa
观测分子量	43-45 kDa, 35 kDa
GenBank蛋白编号	BC001487
基因名称	TDP-43
Gene ID (NCBI)	23435
RRID	AB_2200505
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q13148
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

实验方案

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol
IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol
IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Nature TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A. Authors - Anna-Leigh Brown View Article
human	WB,IHC	Science HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells. Authors - Haiyang Yu KO Validated View Article
human,mouse	IF	Nature TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle. Authors - Thomas O Vogler View Article
mouse	WB,IF	Nature Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. Authors - Lindsay A Becker View Article
human	IF	Nat Med Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis. Authors - Payam Mohassel View Article
human	WB	Nat Cell Biol Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition Authors - Shan Lu View Article

验证数据展示

IHC staining of mouse brain using 12892-1-AP

IF Staining of HeLa using 12892-1-AP

WB analysis of A549 cells using 12892-1-AP

WB analysis of HeLa using 12892-1-AP

IF Staining of mouse brain using 12892-1-AP

IP experiment of HeLa using 12892-1-AP

FC experiment of HeLa using 12892-1-AP

IHC staining of rat brain using 12892-1-AP

IF Staining of mouse brain using 12892-1-AP

TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.

TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle.

Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.

Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis.

Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition