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验证数据展示

  • WB analysis of HeLa using 66499-1-Ig (same clone as 66499-1-PBS)

    WB analysis of HeLa using 66499-1-Ig (same clone as 66499-1-PBS)

    WB result of TAU antibody (66499-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAU transfected HeLa cells. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

    WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

    WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

    IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

    IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TAU Monoclonal antibody, PBS Only

TAU Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66499-1-PBS

产品说明书

CloneNo. 1E9A8

宿主/亚型

Mouse / IgG2c

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66499-1-PBS targets TAU in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 TAU fusion protein Ag21926 种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 microtubule-associated protein tau
别名 DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU
计算分子量 37-46, 79-81 kDa
观测分子量 100 kDa
GenBank蛋白编号BC000558
基因名称 TAU
Gene ID (NCBI) 4137
RRIDAB_2881863
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10636
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (PMID:8752131,15965697, 12485403).Present monoclonal anti-Tau antibody can detect approx 100-kDa bands in brain tissues.