TBX21/T-bet Polyclonal antibody

TBX21/T-bet Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13700-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, FC (Intra), IP, ELISA and More (2)

TBX21, TBX21,T-bet, T bet, T-box protein 21, T-box transcription factor TBX21

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inNK-92 cells, A549 cells, mouse thymus tissue, RAW 264.7 cells
Positive IP detected inmouse thymus tissue
Positive IF/ICC detected inNK-92 cells
Positive FC (Intra) detected inNK92

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13700-1-AP targets TBX21/T-bet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 TBX21/T-bet fusion protein Ag4618 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 T-box 21
别名 TBX21, TBX21,T-bet, T bet, T-box protein 21, T-box transcription factor TBX21
计算分子量 535 aa, 58 kDa
观测分子量 60-62 kDa
GenBank蛋白编号BC039739
基因名称 TBX21/T-bet
Gene ID (NCBI) 30009
ENSEMBL Gene IDENSG00000073861
RRIDAB_10666436
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UL17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

实验方案

Product Specific Protocols
WB protocol for TBX21/T-bet antibody 13700-1-APDownload protocol
IF protocol for TBX21/T-bet antibody 13700-1-APDownload protocol
IP protocol for TBX21/T-bet antibody 13700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.

Authors - Rui Yang
  • KD Validated
ratWB

Int Immunopharmacol

Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway

Authors - Wang Kun
humanWB

Int J Cardiol

Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.

Authors - Lu Yongguang Y
mouseWB

Mol Nutr Food Res

A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways

Authors - Qianye Zhang
ratWB

Biomed Pharmacother

Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

Authors - Yu Xia
humanWB

Front Pharmacol

Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy.

Authors - Weizhen Li
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