TCEB2/Elongin-B Polyclonal antibody
TCEB2/Elongin-B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, K-562 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
产品信息
10779-1-AP targets TCEB2/Elongin-B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | TCEB2/Elongin-B fusion protein Ag1228 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B) |
Synonyms | ELOB, Elongin 18 kDa subunit, Elongin B, SIII, SIII p18, TCEB2 |
Calculated Molecular Weight | 13 kDa |
Observed Molecular Weight | 18 kDa |
GenBank Accession Number | BC013306 |
Gene Symbol | TCEB2 |
Gene ID (NCBI) | 6923 |
RRID | AB_2240375 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q15370 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TCEB2/Elongin-B antibody 10779-1-AP | Download protocol |
IHC protocol for TCEB2/Elongin-B antibody 10779-1-AP | Download protocol |
IP protocol for TCEB2/Elongin-B antibody 10779-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation | ||
Cell Death Differ CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation. | ||
Cell Signal pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation. | ||
bioRxiv The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation | ||
Cell Death Differ USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A | ||