TDP-43 Monoclonal antibody

TDP-43 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1G4F5

Cat no : 66734-1-Ig

Print datasheet

Synonyms

TARDBP, TDP 43, TDP43, TAR DNA-binding protein 43, TAR DNA binding protein 43



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, C6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66734-1-Ig targets TDP-43 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen fusion protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TAR DNA binding protein
Synonyms TARDBP, TDP 43, TDP43, TAR DNA-binding protein 43, TAR DNA binding protein 43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.

实验方案

Product Specific Protocols
WB protocol for TDP-43 antibody 66734-1-IgDownload protocol
IHC protocol for TDP-43 antibody 66734-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Acta Neuropathol

Neurons selectively targeted in frontotemporal dementia reveal early stage TDP-43 pathobiology.

Authors - Alissa L Nana
mouseWB, IF

Int J Biol Sci

Spreading of pathological TDP-43 along corticospinal tract axons induces ALS-like phenotypes in Atg5+/- mice.

Authors - Rui Zhang
mouseIHC

Exp Neurol

Muscle-dominant wild-type TDP-43 expression induces myopathological changes featuring tubular aggregates and TDP-43-positive inclusions.

Authors - Nozomu Tawara
mouseIF

Sci Rep

A microfluidic approach to rescue ALS motor neuron degeneration using rapamycin.

Authors - Phaneendra Chennampally
human

Nat Commun

TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression

Authors - Marta Garcia-Montojo