TDP-43 Monoclonal antibody

TDP-43 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66734-1-Ig

产品说明书

CloneNo. 1G4F5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

TARDBP, TDP 43, TDP43, TAR DNA-binding protein 43, TAR DNA binding protein 43

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, C6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66734-1-Ig targets TDP-43 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 fusion protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TAR DNA binding protein
别名 TARDBP, TDP 43, TDP43, TAR DNA-binding protein 43, TAR DNA binding protein 43
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC001487
基因名称 TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882084
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13148
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.

实验方案

Product Specific Protocols
WB protocol for TDP-43 antibody 66734-1-IgDownload protocol
IHC protocol for TDP-43 antibody 66734-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Acta Neuropathol

Neurons selectively targeted in frontotemporal dementia reveal early stage TDP-43 pathobiology.

Authors - Alissa L Nana
mouseWB, IF

Int J Biol Sci

Spreading of pathological TDP-43 along corticospinal tract axons induces ALS-like phenotypes in Atg5+/- mice.

Authors - Rui Zhang
mouseIHC

Exp Neurol

Muscle-dominant wild-type TDP-43 expression induces myopathological changes featuring tubular aggregates and TDP-43-positive inclusions.

Authors - Nozomu Tawara
mouseIF

Sci Rep

A microfluidic approach to rescue ALS motor neuron degeneration using rapamycin.

Authors - Phaneendra Chennampally
human

Nat Commun

TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression

Authors - Marta Garcia-Montojo
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