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  • Featured Product
  • KD/KO Validated

TH Monoclonal antibody, PBS Only

TH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H7B7

Cat no : 66334-1-PBS

Print datasheet

Synonyms

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

验证数据展示

  • WB analysis of PC-12 using 66334-1-Ig (same clone as 66334-1-PBS)

    WB analysis of PC-12 using 66334-1-Ig (same clone as 66334-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    IF Staining of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TH Ready-To-Use IHC Kit. TH primary antibody included.

产品信息

66334-1-PBS targets TH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TH fusion protein Ag23075 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tyrosine hydroxylase
Synonyms TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase
Calculated Molecular Weight 528 aa, 59 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC104967
Gene Symbol TH
Gene ID (NCBI) 7054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07101
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.