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验证数据展示

  • WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    WB result of TLR2 antibody (66645-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR2 transfected THP-1 cells. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

    IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66645-1-Ig (TLR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR2 antibody (66645-1-Ig, Clone: 1C1D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50388-1

    Cytometric bead array standard curve of MP50388-1

    Cytometric bead array standard curve of MP50388-1, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-2-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50388-3

    Cytometric bead array standard curve of MP50388-3

    Cytometric bead array standard curve of MP50388-3, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-4-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO验证

TLR2 Monoclonal antibody, PBS Only (Capture)

TLR2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66645-1-PBS

产品说明书

CloneNo. 1C1D3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4, toll like receptor 2, TIL4, CD282

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66645-1-PBS targets TLR2 as part of a matched antibody pair:

MP50388-1: 66645-1-PBS capture and 66645-2-PBS detection (validated in Cytometric bead array)

MP50388-3: 66645-1-PBS capture and 66645-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 TLR2 fusion protein Ag25626 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 toll-like receptor 2
别名 Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4, toll like receptor 2, TIL4, CD282
计算分子量 784 aa, 90 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC033756
基因名称 TLR2
Gene ID (NCBI) 7097
RRIDAB_2882003
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60603
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).