TLR3 Polyclonal antibody

TLR3 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

FC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 17766-1-AP

Print datasheet

Synonyms

CD283, TLR3, toll like receptor 3



经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 1 publications below
IHCSee 6 publications below

产品信息

The immunogen of 17766-1-AP is TLR3 Fusion Protein expressed in E. coli.

Tested Applications FC, IHC, ELISA
Cited Applications IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen TLR3 fusion protein Ag12171 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name toll-like receptor 3
Synonyms CD283, TLR3, toll like receptor 3
Calculated Molecular Weight 904 aa, 104 kDa
GenBank Accession NumberBC094737
Gene Symbol TLR3
Gene ID (NCBI) 7098
RRIDAB_2878436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15455
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR3 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production and dendritic cell (DC) maturation (PMID: 18262679).

实验方案

Product Specific Protocols
IHC protocol for TLR3 antibody 17766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC

J Mater Chem B

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating

Authors - Bin Yan
human

J Cell Mol Med

Down-regulation of miR-10a-5p in synoviocytes contributes to TBX5-controlled joint inflammation.

Authors - Nazim Hussain
mouse

Phytomedicine

Wu-Mei-Wan prevents high-fat diet-induced obesity by reducing white adipose tissue and enhancing brown adipose tissue function.

Authors - Fan Wu
human

Front Cell Infect Microbiol

Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.

Authors - Matthew Charman
human

RNA

MiR-146a down-regulates inflammatory response by targeting TLR3 and TRAF6 in Coxsackievirus B infection.

Authors - Yanru Fei
mouse

J Appl Toxicol

Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers.

Authors - Xiaomin Tang