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  • Featured Product
  • KD/KO Validated

TLR4 Monoclonal antibody, PBS Only

TLR4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G9A4

Cat no : 66350-1-PBS

Print datasheet

Synonyms

toll like receptor 4, TOLL, hToll, CD284, ARMD10

验证数据展示

  • WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

产品信息

66350-1-PBS targets TLR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen TLR4 fusion protein Ag13857 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name toll-like receptor 4
Synonyms toll like receptor 4, TOLL, hToll, CD284, ARMD10
Calculated Molecular Weight 839 aa, 96 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC117422
Gene Symbol TLR4
Gene ID (NCBI) 7099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00206
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.