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验证数据展示

  • WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TLR4 Monoclonal antibody, PBS Only

TLR4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66350-1-PBS

产品说明书

CloneNo. 3G9A4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

toll like receptor 4, TOLL, hToll, CD284, ARMD10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66350-1-PBS targets TLR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TLR4 fusion protein Ag13857 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 toll-like receptor 4
别名 toll like receptor 4, TOLL, hToll, CD284, ARMD10
计算分子量 839 aa, 96 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC117422
基因名称 TLR4
Gene ID (NCBI) 7099
RRIDAB_2881730
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00206
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.