• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TLR5 Monoclonal antibody, PBS Only

TLR5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4B3D2

Cat no : 66570-1-PBS

Print datasheet

Synonyms

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

验证数据展示

  • IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    FC experiment of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TLR5 (66570-1-Ig, Clone:4B3D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66570-1-Ig (same clone as 66570-1-PBS)

    WB analysis of A549 using 66570-1-Ig (same clone as 66570-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

产品信息

66570-1-PBS targets TLR5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity pig, rat, mouse, human
Immunogen TLR5 fusion protein Ag13840 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name toll-like receptor 5
Synonyms Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1
Calculated Molecular Weight 858 aa, 98 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC109119
Gene Symbol TLR5
Gene ID (NCBI) 7100
RRIDAB_2881931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60602
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.