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Host
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Species Reactivity
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Species Reactivity
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Compatible Species
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Conjugate
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验证数据展示

  • IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    FC experiment of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TLR5 (66570-1-Ig, Clone:4B3D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66570-1-Ig (same clone as 66570-1-PBS)

    WB analysis of A549 using 66570-1-Ig (same clone as 66570-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

TLR5 Monoclonal antibody, PBS Only

TLR5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66570-1-PBS

产品说明书

CloneNo. 4B3D2

宿主/亚型

Mouse / IgG1

种属反应性

pig, rat, mouse, human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66570-1-PBS targets TLR5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with pig, rat, mouse, human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 pig, rat, mouse, human
免疫原 TLR5 fusion protein Ag13840 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 toll-like receptor 5
别名 Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1
计算分子量 858 aa, 98 kDa
观测分子量 100 kDa
GenBank蛋白编号BC109119
基因名称 TLR5
Gene ID (NCBI) 7100
RRIDAB_2881931
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60602
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.