验证数据展示

IHC staining of human lung using 17232-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17232-1-AP (TLR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of RAW 264.7 using 17232-1-AP
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TLR7 antibody (17232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
WB analysis of mouse lung using 17232-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 17232-1-AP (TLR7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17232-1-AP
WB result of TLR7 antibody (17232-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR7 transfected Jurkat cells.
IHC staining of human small intestine using 17232-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO验证

TLR7 Polyclonal antibody

TLR7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17232-1-AP

产品说明书

引用文献(13)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

UNQ248/PRO285, Toll-like receptor 7, toll like receptor 7

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (13)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse lung tissue, Jurkat cells
Positive IHC detected in	human lung tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	RAW 264.7 cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 4 publications below
IF	See 1 publications below

产品信息

17232-1-AP targets TLR7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	TLR7 fusion protein Ag11073 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	toll-like receptor 7
别名	UNQ248/PRO285, Toll-like receptor 7, toll like receptor 7
计算分子量	1049 aa, 121 kDa
观测分子量	121 kDa, 100 kDa
GenBank蛋白编号	BC033651
基因名称	TLR7
Gene ID (NCBI)	51284
RRID	AB_2203464
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9NYK1
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Among the TLR family, TLR7 (toll-like receptor 7) is an endosomal innate immune sensor that can detect single-stranded ribonucleic acid. Constitutive expression of TLR7 is pre-dominant in dendritic cells and B cells compared to other immune cells. Low levels of TLR7 expression have been observed in non-immune cells, such as hepatocytes, epithelial cells, and keratinocytes (PMID: 37596656).

实验方案

Product Specific Protocols
WB protocol for TLR7 antibody 17232-1-AP	Download protocol
IHC protocol for TLR7 antibody 17232-1-AP	Download protocol
IF protocol for TLR7 antibody 17232-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IHC	Nat Commun TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase Authors - Gang Liu View Article
rat	WB	Acta Physiol (Oxf) Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion. Authors - Li Zhang View Article
human	WB	Stem Cell Res Ther Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7. Authors - Ruijie Zhang KD Validated View Article
mouse	WB,IF	Mol Neurobiol Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment Authors - Liyun Deng View Article
mouse	WB	Front Immunol Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations. Authors - Lujie Yang View Article
rat	WB	Mol Cell Endocrinol Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats. Authors - Dongmin Li View Article

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验证数据展示

IHC staining of human lung using 17232-1-AP

IF Staining of RAW 264.7 using 17232-1-AP

WB analysis of mouse lung using 17232-1-AP

WB analysis of Jurkat using 17232-1-AP

IHC staining of human small intestine using 17232-1-AP

TLR7 Polyclonal antibody

TLR7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.

Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment

Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.