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  • KD/KO Validated

TLR7 Polyclonal antibody

TLR7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17232-1-AP

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Synonyms

TLR7, toll like receptor 7



经过测试的应用

Positive WB detected inmouse lung tissue, Jurkat cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17232-1-AP targets TLR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen TLR7 fusion protein Ag11073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name toll-like receptor 7
Synonyms TLR7, toll like receptor 7
Calculated Molecular Weight 1049 aa, 121 kDa
Observed Molecular Weight 121 kDa, 100 kDa
GenBank Accession NumberBC033651
Gene Symbol TLR7
Gene ID (NCBI) 51284
RRIDAB_2203464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYK1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Among the TLR family, TLR7 (toll-like receptor 7) is an endosomal innate immune sensor that can detect single-stranded ribonucleic acid. Constitutive expression of TLR7 is pre-dominant in dendritic cells and B cells compared to other immune cells. Low levels of TLR7 expression have been observed in non-immune cells, such as hepatocytes, epithelial cells, and keratinocytes (PMID: 37596656).

实验方案

Product Specific Protocols
WB protocol for TLR7 antibody 17232-1-APDownload protocol
IHC protocol for TLR7 antibody 17232-1-APDownload protocol
IF protocol for TLR7 antibody 17232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Commun

TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase

Authors - Gang Liu
ratWB

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang
humanWB

Stem Cell Res Ther

Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.

Authors - Ruijie Zhang
  • KD Validated
mouseWB,IF

Mol Neurobiol

Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment

Authors - Liyun Deng
mouseWB

Front Immunol

Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.

Authors - Lujie Yang
ratWB

Mol Cell Endocrinol

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.

Authors - Dongmin Li