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TLR8 Monoclonal antibody, PBS Only

TLR8 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3B6A4

Cat No : 67317-1-PBS

Print datasheet

Synonyms

CD288, TLR8, toll like receptor 8

验证数据展示

  • IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • WB analysis using 67317-1-Ig (same clone as 67317-1-PBS)

    WB analysis using 67317-1-Ig (same clone as 67317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

产品信息

67317-1-PBS targets TLR8 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen TLR8 fusion protein Ag16566 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name toll-like receptor 8
Synonyms CD288, TLR8, toll like receptor 8
Calculated Molecular Weight 1041 aa, 120 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC101075
Gene Symbol TLR8
Gene ID (NCBI) 51311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NR97
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.