TMEM119 Polyclonal antibody

TMEM119 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 27585-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, FC (Intra), ELISA and More (2)

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格: 

-/ -


经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue, mouse liver tissue
Positive FC (Intra) detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27585-1-AP targets TMEM119 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 TMEM119 fusion protein Ag26269 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 119
别名 UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF
计算分子量 29 kDa
观测分子量 45 kDa
GenBank蛋白编号NM_181724
基因名称 TMEM119
Gene ID (NCBI) 338773
RRIDAB_2880915
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4V9L6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.

实验方案

Product Specific Protocols
IHC protocol for TMEM119 antibody 27585-1-APDownload protocol
IF protocol for TMEM119 antibody 27585-1-APDownload protocol
FC protocol for TMEM119 antibody 27585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Sci Adv

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Authors - Christian F Guerrero-Juarez
mouse,humanFC

Nat Commun

P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression.

Authors - Eilam Yeini
humanIF

Glia

Transmembrane protein 119 is neither a specific nor a reliable marker for microglia.

Authors - Elise Vankriekelsvenne
mouseIF

Pharmacol Res

MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.

Authors - Danqing Xin
mouseIF

Front Immunol

Didymin Suppresses Microglia Pyroptosis and Neuroinflammation Through the Asc/Caspase-1/GSDMD Pathway Following Experimental Intracerebral Hemorrhage.

Authors - Lingui Gu
human

Neurochem Res

BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway

Authors - Xiaojin Wei
Loading...