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- KD/KO Validated
ASC/TMS1 Polyclonal antibody
ASC/TMS1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
247
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, A549 cells, Raji cells, U-937 cells, THP-1 cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ASC/TMS1 Ready-To-Use IHC Kit. ASC/TMS1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10500-1-AP targets ASC/TMS1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, ELISA |
| Tested Reactivity | human |
| Cited Reactivity | human, pig, canine, bovine |
| Immunogen | ASC/TMS1 fusion protein Ag0774 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PYD and CARD domain containing |
| Synonyms | PYCARD, hASC, Caspase recruitment domain-containing protein 5, CARD5, ASC |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC004470 |
| Gene Symbol | ASC/TMS1 |
| Gene ID (NCBI) | 29108 |
| RRID | AB_2174862 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| IHC protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| IF protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| IP protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis | ||
Transl Res IMM-H004 therapy for permanent focal ischemic cerebral injury via CKLF1/CCR4-mediated NLRP3 inflammasome activation. | ||
Environ Pollut Disturbance of Mitochondrial Dynamics Led to Spermatogenesis Disorder in Mice Exposed to Polystyrene Micro- and Nanoplastics | ||
Cancer Res Silencing of TMS1/ASC promotes resistance to anoikis in breast epithelial cells.
| ||
Cancer Res Role of hMOF-dependent histone H4 lysine 16 acetylation in the maintenance of TMS1/ASC gene activity. | ||
Cell Mol Life Sci circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells |
