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TNC/Tenascin-C Polyclonal antibody

TNC/Tenascin-C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27789-1-AP

Print datasheet

Synonyms

Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC

验证数据展示

  • WB analysis using 27789-1-AP

    WB analysis using 27789-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 27789-1-AP (TNC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27789-1-AP

    IHC staining of human liver cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 27789-1-AP

    IHC staining of human liver cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27789-1-AP targets TNC/Tenascin-C in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen TNC/Tenascin-C fusion protein Ag27122 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tenascin C
Synonyms Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC
Calculated Molecular Weight 2201 aa, 241 kDa
GenBank Accession NumberBC151843
Gene Symbol TNC
Gene ID (NCBI) 3371
RRIDAB_2918131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24821
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

实验方案

Product Specific Protocols
WB protocol for TNC/Tenascin-C antibody 27789-1-APDownload protocol
IHC protocol for TNC/Tenascin-C antibody 27789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols