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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TNFR1/CD120a Monoclonal antibody

TNFR1/CD120a Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A6E3

Cat no : CL488-60192

Print datasheet

Synonyms

TNFRSF1A, p60, p55 R, p55, FPF

验证数据展示

  • IF Staining of human liver cancer using CL488-60192

    IF Staining of human liver cancer using CL488-60192

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 TNFR1/CD120a antibody (CL488-60192, Clone: 2A6E3 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL488-60192

    IF Staining of human liver cancer using CL488-60192

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 TNFR1/CD120a antibody (CL488-60192, Clone: 2A6E3 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60192-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60192 targets TNFR1/CD120a in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen TNFR1/CD120a fusion protein Ag16112 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 1A
Synonyms TNFRSF1A, p60, p55 R, p55, FPF
Calculated Molecular Weight 455 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC010140
Gene Symbol TNFR1
Gene ID (NCBI) 7132
RRIDAB_2919235
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19438
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TNFR1/CD120a antibody CL488-60192Download protocol
Standard Protocols
Click here to view our Standard Protocols