验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18409-1-AP targets TOMM40 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, monkey, bovine |
免疫原 | TOMM40 fusion protein Ag13065 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | translocase of outer mitochondrial membrane 40 homolog (yeast) |
别名 | TOM40, PER-EC1, PEREC1, PER EC1, p38.5 |
计算分子量 | 38 kDa |
观测分子量 | 38 kDa |
GenBank蛋白编号 | BC017224 |
基因名称 | TOMM40 |
Gene ID (NCBI) | 10452 |
RRID | AB_2303725 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O96008 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
实验方案
Product Specific Protocols | |
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WB protocol for TOMM40 antibody 18409-1-AP | Download protocol |
IHC protocol for TOMM40 antibody 18409-1-AP | Download protocol |
IF protocol for TOMM40 antibody 18409-1-AP | Download protocol |
IP protocol for TOMM40 antibody 18409-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. | ||
Cell Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. | ||
Cell Discov The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth | ||
Cell Metab Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
| ||
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control. |