TOP1 Polyclonal antibody

TOP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20705-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

Top-I, TOPI, EC:5.6.2.1, DNA topoisomerase I, DNA topoisomerase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20705-1-AP targets TOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 topoisomerase (DNA) I
别名 Top-I, TOPI, EC:5.6.2.1, DNA topoisomerase I, DNA topoisomerase 1
计算分子量 91 kDa
观测分子量 90 kDa
GenBank蛋白编号NM_003286
基因名称 TOP1
Gene ID (NCBI) 7150
RRIDAB_10695762
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11387
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.

实验方案

Product Specific Protocols
WB protocol for TOP1 antibody 20705-1-APDownload protocol
IHC protocol for TOP1 antibody 20705-1-APDownload protocol
IF protocol for TOP1 antibody 20705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.

Authors - Bo Zhao
humanWB, IF

Cell Chem Biol

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Authors - Amy Flor
humanWB

J Transl Med

Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1

Authors - Ruijiao Chen
humanWB

Oncotarget

Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.

Authors - Xia Lingzi
humanWB

J Cell Mol Med

EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.

Authors - Wenbing Wu
mouseWB

iScience

Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.

Authors - Xue Hao
Loading...