• Featured Product
  • KD/KO验证

TOP2A-Specific Polyclonal antibody

TOP2A-Specific Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 20233-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA and More (1)

TOP2A, TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20233-1-AP targets TOP2A-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 topoisomerase (DNA) II alpha 170kDa
别名 TOP2A, TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha
计算分子量 174 kDa
观测分子量 170-177 kDa
GenBank蛋白编号NM_001067
基因名称 TOP2A
Gene ID (NCBI) 7153
RRIDAB_10664923
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11388
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

实验方案

Product Specific Protocols
WB protocol for TOP2A-Specific antibody 20233-1-APDownload protocol
IHC protocol for TOP2A-Specific antibody 20233-1-APDownload protocol
IP protocol for TOP2A-Specific antibody 20233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

Authors - Anand Minajigi
mouseWB

Nat Commun

Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability

Authors - Zaili Luo
mouseIHC

Research (Wash D C)

Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation

Authors - Chao Song
humanWB

J Cell Biol

LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.

Authors - Yilin Fan
humanWB

Oncotarget

TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.

Authors - Xuefeng Li
  • KD Validated
humanWB

J Med Chem

Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.

Authors - Xiaohong Yang
Loading...