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TRAIL Polyclonal antibody

TRAIL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27064-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman milk
Positive IHC detected inhuman kidney tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27064-1-AP targets TRAIL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 TRAIL fusion protein Ag25746 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 10
别名 TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253
计算分子量 281 aa, 33 kDa
观测分子量 33 kDa, 20 kDa
GenBank蛋白编号BC032722
基因名称 TRAIL
Gene ID (NCBI) 8743
RRIDAB_2880737
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50591
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

实验方案

Product Specific Protocols
WB protocol for TRAIL antibody 27064-1-APDownload protocol
IHC protocol for TRAIL antibody 27064-1-APDownload protocol
IF protocol for TRAIL antibody 27064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Stem Cells Transl Med

Site-Specific Integration of TRAIL in iPSC-Derived Mesenchymal Stem Cells for Targeted Cancer Therapy.

Authors - Zujia Wang
humanWB

Int J Pharm

Fusion of an EGFR-antagonistic affibody enhances the anti-tumor effect of TRAIL to EGFR positive tumors.

Authors - Dianlong Jia
humanWB

Arthritis Res Ther

IL13Rα1 protects against rheumatoid arthritis by combating the apoptotic resistance of fibroblast-like synoviocytes.

Authors - Xiaomei Yang
humanWB

Cell Biosci

Long noncoding RNA TANCR promotes γδ T cells activation by regulating TRAIL expression in cis.

Authors - Chuan Yang
  • KD Validated
humanWB

J Nat Prod

Sanggenon C Suppresses Tumorigenesis of Gastric Cancer by Blocking ERK-Drp1-Mediated Mitochondrial Fission

Authors - Xiao-Jie Chen
humanWB

Phytomedicine

Evodiamine inhibits ESCC by inducing M-phase cell-cycle arrest via CUL4A/p53/p21 axis and activating noxa-dependent intrinsic and DR4-dependent extrinsic apoptosis

Authors - Li Zhang
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