验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13623-1-AP targets TRIM15 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IP, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | TRIM15 fusion protein Ag4533 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | tripartite motif-containing 15 |
别名 | RING finger protein 93, RNF93, TRIM15, tripartite motif containing 15, Zinc finger protein 178, Zinc finger protein B7, ZNF178, ZNFB7 |
计算分子量 | 465 aa, 52 kDa |
观测分子量 | 52 kDa |
GenBank蛋白编号 | BC038585 |
基因名称 | TRIM15 |
Gene ID (NCBI) | 89870 |
RRID | AB_2303892 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9C019 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM15 (Tripartite motif-containing protein 15) is also named as RNF93, ZNF178, ZNFB7 and belongs to the TRIM/RBCC family. It acts as an ubiquitin-protein ligase and contributes to the endogenous restriction of retroviruses in cells.
实验方案
Product Specific Protocols | |
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WB protocol for TRIM15 antibody 13623-1-AP | Download protocol |
IHC protocol for TRIM15 antibody 13623-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Cell Biol TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.
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Biochim Biophys Acta Mol Basis Dis Ubiquitin ligase TRIM15 promotes the progression of pancreatic cancer via the upregulation of the IGF2BP2-TLR4 axis | ||
J Cell Sci TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly.
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iScience Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma | ||
J Virol TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.
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J Cancer Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses. |