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TRIM5 Polyclonal antibody

TRIM5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11476-1-AP

Print datasheet

Synonyms

RING finger protein 88, RNF88, TRIM5, TRIM5alpha, tripartite motif containing 5

验证数据展示

  • WB analysis of HeLa using 11476-1-AP

    WB analysis of HeLa using 11476-1-AP

    WB result of TRIM5 antibody (11476-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM5 transfected HeLa cells.

  • WB analysis using 11476-1-AP

    WB analysis using 11476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 11476-1-AP

    IHC staining of mouse liver using 11476-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 11476-1-AP

    IHC staining of mouse liver using 11476-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, MKN-45 cells
Positive IHC detected inmouse liver tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11476-1-AP targets TRIM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TRIM5 fusion protein Ag1973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 5
Synonyms RING finger protein 88, RNF88, TRIM5, TRIM5alpha, tripartite motif containing 5
Calculated Molecular Weight 493 aa, 56 kDa
Observed Molecular Weight 46 kDa, 56 kDa
GenBank Accession NumberBC021258
Gene Symbol TRIM5
Gene ID (NCBI) 85363
RRIDAB_2877767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C035
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM5 belongs to the large tripartite motif protein family, members of which typically are composed of 3 zinc-binding domains, a RING, unique B-box type 1 and B-box type 2 domains, followed by a coiled-coil (CC) region. TRIM proteins use homomultimerization to identify specific cell compartments. TRIM5 promotes innate immune signaling and that this activity is amplified by retroviral infection and interaction with the capsid lattice. TRIM5 has 6 variants termed alpha, beta, gamma, delta, epsilon, and iota with the MW of 56 kDa, 46 kDa, 40 kDa, 37 kDa, 31 kDa and 29 kDa. TRIM5 can form homodimers (~70 kDa) and homotrimers (90-160 kDa).

实验方案

Product Specific Protocols
WB protocol for TRIM5 antibody 11476-1-APDownload protocol
IHC protocol for TRIM5 antibody 11476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols