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  • Featured Product
  • KD/KO Validated

HTF9C Polyclonal antibody

HTF9C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16199-1-AP

Print datasheet

Synonyms

HTF9C, TRMT2A

验证数据展示

  • IHC staining of human kidney using 16199-1-AP

    IHC staining of human kidney using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 16199-1-AP

    IHC staining of human kidney using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of MCF-7 using 16199-1-AP

    WB analysis of MCF-7 using 16199-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16199-1-AP targets HTF9C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HTF9C fusion protein Ag9112 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
Synonyms HTF9C, TRMT2A
Calculated Molecular Weight 625 aa, 69 kDa
Observed Molecular Weight 42-44 kDa, 68-75 kDa
GenBank Accession NumberBC013352
Gene Symbol TRMT2A
Gene ID (NCBI) 27037
RRIDAB_2209623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZ69
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

实验方案

Product Specific Protocols
WB protocol for HTF9C antibody 16199-1-APDownload protocol
IHC protocol for HTF9C antibody 16199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

Authors - Wei Dai
  • KD Validated
View Article