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TRPM1 Polyclonal antibody

TRPM1 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 55111-1-AP

Print datasheet

Synonyms

LTRPC1, Melastatin 1, MLSN, MLSN1, TRPM1

验证数据展示

  • IF Staining of SH-SY5Y using 55111-1-AP

    IF Staining of SH-SY5Y using 55111-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 55111-1-AP (TRPM1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human lung cancer using 55111-1-AP

    IHC staining of human lung cancer using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55111-1-AP (TRPM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 55111-1-AP

    IHC staining of human lung cancer using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55111-1-AP (TRPM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of SH-SY5Y using 55111-1-AP

    FC experiment of SH-SY5Y using 55111-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug TRPM1 antibody (55111-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • IHC staining of human malignant melanoma using 55111-1-AP

    IHC staining of human malignant melanoma using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55111-1-AP (TRPM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human malignant melanoma using 55111-1-AP

    IHC staining of human malignant melanoma using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55111-1-AP (TRPM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, A431 cells
Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:25-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55111-1-AP targets TRPM1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily M, member 1
Synonyms LTRPC1, Melastatin 1, MLSN, MLSN1, TRPM1
Calculated Molecular Weight 182 kDa
GenBank Accession NumberNM_002420
Gene Symbol TRPM1
Gene ID (NCBI) 4308
RRIDAB_2881269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4N2
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TRPM1 antibody 55111-1-APDownload protocol
IF protocol for TRPM1 antibody 55111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols