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  • KD/KO Validated

TSG101 Polyclonal antibody

TSG101 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IF, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14497-1-AP

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Synonyms

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23



经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14497-1-AP targets TSG101 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, monkey, bovine
Immunogen TSG101 fusion protein Ag5920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor susceptibility gene 101
Synonyms ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
RRIDAB_2208090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99816
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

实验方案

Product Specific Protocols
WB protocol for TSG101 antibody 14497-1-APDownload protocol
IHC protocol for TSG101 antibody 14497-1-APDownload protocol
IF protocol for TSG101 antibody 14497-1-APDownload protocol
IP protocol for TSG101 antibody 14497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB, IHC

Nature

Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.

Authors - Lin Zhang
humanWB

Cell

Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.

Authors - Mirjam C Boelens
humanWB

Cell

Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.

Authors - Barzin Y Nabet
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
  • KD Validated
mouse,humanWB

ACS Nano

Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.

Authors - Meng Zhao