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验证数据展示

  • WB analysis of MCF-7 using 17088-1-AP

    WB analysis of MCF-7 using 17088-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17088-1-AP (TUBGCP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 17088-1-AP

    IHC staining of human placenta using 17088-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17088-1-AP (TUBGCP4 antibody) at dilution of 1:100 (under 10x lens).

TUBGCP4 Polyclonal antibody

TUBGCP4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17088-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

76P, GCP 4, GCP4, h76p, hGCP4, hGrip76, TUBGCP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17088-1-AP targets TUBGCP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TUBGCP4 fusion protein Ag9976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, gamma complex associated protein 4
Synonyms 76P, GCP 4, GCP4, h76p, hGCP4, hGrip76, TUBGCP4
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 76 kDa
GenBank Accession NumberBC000966
Gene Symbol TUBGCP4
Gene ID (NCBI) 27229
RRIDAB_2211283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TUBGCP4 antibody 17088-1-APDownload protocol
IHC protocol for TUBGCP4 antibody 17088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols