TWIST1 Polyclonal antibody
TWIST1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, MCF-7 cells, SGC-7901 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18125-1-AP targets TWIST1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | TWIST1 fusion protein Ag12700 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | twist homolog 1 (Drosophila) |
| Synonyms | ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1 |
| Calculated Molecular Weight | 202 aa, 21 kDa |
| Observed Molecular Weight | 22 kDa, 28 kDa, 45-60 kDa |
| GenBank Accession Number | BC036704 |
| Gene Symbol | TWIST1 |
| Gene ID (NCBI) | 7291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15672 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TWIST1, a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. It is also implicated in tumorigenesis, invasion and metastasis. TWIST1 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TWIST1 antibody 18125-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis. | ||
Int J Biochem Cell Biol Blue light emitting diodes irradiation causes cell death in colorectal cancer by inducing ROS production and DNA damage. | ||
Cancer Lett Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry. | ||
Int J Cancer Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer. | ||
Sci Rep Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model. |
