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验证数据展示

  • WB analysis of HeLa using 66362-1-Ig (same clone as 66362-1-PBS)

    WB analysis of HeLa using 66362-1-Ig (same clone as 66362-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 66362-1-Ig (same clone as 66362-1-PBS)

    IF Staining of MDCK using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

Tubulin Beta Class V Monoclonal antibody, PBS Only

Tubulin Beta Class V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66362-1-PBS

CloneNo.

1D7B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, Canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66362-1-PBS targets Tubulin Beta Class V in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, Canine samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, Canine
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tubulin, beta 6
Synonyms HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_032525
Gene Symbol Beta Tubulin
Gene ID (NCBI) 84617
RRIDAB_2881742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.