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验证数据展示

  • WB analysis of HeLa using 66362-1-Ig (same clone as 66362-1-PBS)

    WB analysis of HeLa using 66362-1-Ig (same clone as 66362-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 66362-1-Ig (same clone as 66362-1-PBS)

    IF Staining of MDCK using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

Tubulin Beta Class V Monoclonal antibody, PBS Only

Tubulin Beta Class V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66362-1-PBS

产品说明书

CloneNo. 1D7B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, Canine

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66362-1-PBS targets Tubulin Beta Class V in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, Canine samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, Canine
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, beta 6
别名 HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号NM_032525
基因名称 Beta Tubulin
Gene ID (NCBI) 84617
RRIDAB_2881742
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.