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U2AF2/U2AF65 Polyclonal antibody

U2AF2/U2AF65 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15624-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, ELISA and More (2)

hU2AF(65), hU2AF65, U2AF2, U2AF65

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HEK-293T cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy U2AF2 Ready-To-Use IHC Kit. U2AF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15624-1-AP targets U2AF2/U2AF65 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, RIP, IHC
经测试反应性 human
文献引用反应性human, mouse, bovine
免疫原 U2AF2/U2AF65 fusion protein Ag8070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 U2 small nuclear RNA auxiliary factor 2
别名 hU2AF(65), hU2AF65, U2AF2, U2AF65
计算分子量 54 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC008740
基因名称 U2AF2
Gene ID (NCBI) 11338
RRIDAB_2211330
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP26368
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

实验方案

Product Specific Protocols
WB protocol for U2AF2/U2AF65 antibody 15624-1-APDownload protocol
IHC protocol for U2AF2/U2AF65 antibody 15624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
mouseIF,RIP

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong
humanWB

Sci Rep

Identification of novel PANDAR protein interaction partners involved in splicing regulation.

Authors - N Pospiech
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
humanWB

J Immunol Res

Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.

Authors - Li Fang
humanWB

Cell Death Dis

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.

Authors - Shicheng Sun
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