验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293T cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15624-1-AP targets U2AF2/U2AF65 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, RIP, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 |
CatNo: Ag8070 Product name: Recombinant human U2AF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 116-471 aa of BC008740 Sequence: IPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | U2 small nuclear RNA auxiliary factor 2 |
| 别名 | hU2AF(65), hU2AF65, U2AF2, U2AF65 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC008740 |
| 基因名称 | U2AF2 |
| Gene ID (NCBI) | 11338 |
| RRID | AB_2211330 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P26368 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for U2AF2/U2AF65 antibody 15624-1-AP | Download protocol |
| IHC protocol for U2AF2/U2AF65 antibody 15624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. | ||
J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. | ||
Sci Rep Identification of novel PANDAR protein interaction partners involved in splicing regulation. | ||
iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. | ||
J Immunol Res Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p. | ||
Cell Death Dis RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner. |
