验证数据展示
经过测试的应用
Positive WB detected in | rat brain tissue, HEK-293 cells, HepG2 cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23449-1-AP targets UBQLN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF, IP |
经测试反应性 | human, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | UBQLN2 fusion protein Ag20149 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ubiquilin 2 |
别名 | CHAP1, CHAP1/DSK2, Dsk2, DSK2 homolog, hPLIC 2, HRIHFB2157, LIC 2, N4BP4, PLIC 2, PLIC2, RIHFB2157, ubiquilin 2, Ubiquilin-2, UBQLN2 |
计算分子量 | 624 aa, 66 kDa |
观测分子量 | 66-70 kDa |
GenBank蛋白编号 | BC069237 |
基因名称 | UBQLN2 |
Gene ID (NCBI) | 29978 |
RRID | AB_2879282 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UHD9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for UBQLN2 antibody 23449-1-AP | Download protocol |
IHC protocol for UBQLN2 antibody 23449-1-AP | Download protocol |
IF protocol for UBQLN2 antibody 23449-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Brain Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies | ||
Hum Mol Genet Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration. | ||
J Neurochem Pathogenic mutation of UBQLN2 impairs its interaction with UBXD8 and disrupts endoplasmic reticulum-associated protein degradation. | ||
F1000Res The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
| ||
bioRxiv Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4 |