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UCP1 Polyclonal antibody

UCP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23673-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, ELISA and More (1)

UCP 1, UCP, Thermogenin, SLC25A7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brown adipose tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse brown adipose tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23673-1-AP targets UCP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, bovine, hamster, goat
免疫原 UCP1 fusion protein Ag20388 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uncoupling protein 1 (mitochondrial, proton carrier)
别名 UCP 1, UCP, Thermogenin, SLC25A7
计算分子量 307 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC098168
基因名称 UCP1
Gene ID (NCBI) 7350
RRIDAB_2828003
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25874
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

实验方案

Product Specific Protocols
WB protocol for UCP1 antibody 23673-1-APDownload protocol
IHC protocol for UCP1 antibody 23673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
  • KO Validated
mouseWB

Nat Commun

Improvement of obesity-associated disorders by a small-molecule drug targeting mitochondria of adipose tissue macrophages.

Authors - Yawei Wang
mouseWB,IHC

Mol Biol Evol

Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans

Authors - Ming Zhou
mouseWB

Diabetes

Exosomes From Adipose-Derived Stem Cells Attenuate Adipose Inflammation and Obesity Through Polarizing M2 Macrophages and Beiging in White Adipose Tissues.

Authors - Hui Zhao
bovineIF

Cell Prolif

Procyanidin B2 improves developmental capacity of bovine oocytes via promoting PPARγ/UCP1-mediated uncoupling lipid catabolism during in vitro maturation

Authors - Yuwen Luo
mouseWB

JCI Insight

Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism

Authors - Benedict Abdon
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