UGCGL1 Polyclonal antibody

UGCGL1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14170-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

UGGT1, UDP-glucose:glycoprotein glucosyltransferase 1, UDP-glucose ceramide glucosyltransferase-like 1, UDP--Glc:glycoprotein glucosyltransferase, hUGT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14170-1-AP targets UGCGL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 UGCGL1 fusion protein Ag5369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-glucose ceramide glucosyltransferase-like 1
别名 UGGT1, UDP-glucose:glycoprotein glucosyltransferase 1, UDP-glucose ceramide glucosyltransferase-like 1, UDP--Glc:glycoprotein glucosyltransferase, hUGT1
计算分子量 1531 aa, 175 kDa
观测分子量 170 kDa
GenBank蛋白编号BC041098
基因名称 UGCGL1
Gene ID (NCBI) 56886
RRIDAB_1288973
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYU2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UDP-glucose:glycoprotein glucosyltransferase 1 (UGCGL1) is a central quality control gatekeeper in the mammalian endoplasmic reticulum (ER) and serves as a folding sensor in the calnexin/calreticulin glycoprotein quality control cycle (PMID: 28425917). UGCGL1 acts as a checkpoint in the quality control of the MHC class I antigen presentation pathway (PMID: 21383159). Western blot analysis detected UGCGL1 at an apparent molecular mass of 170 kDa.

实验方案

Product Specific Protocols
WB protocol for UGCGL1 antibody 14170-1-APDownload protocol
IHC protocol for UGCGL1 antibody 14170-1-APDownload protocol
IP protocol for UGCGL1 antibody 14170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

J Cell Biol

Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins

Authors - Amber M Cheatham
humanWB

Mol Cell Proteomics

Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis

Authors - Madison T. Wright
humanWB

Mol Cell Proteomics

Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.

Authors - Madison T Wright
ratIF,WB

Toxicology

Tauroursodeoxycholic acid attenuates cisplatin-induced ototoxicity by inhibiting the accumulation and aggregation of unfolded or misfolded proteins in the endoplasmic reticulum.

Authors - Yingying Wen
humanWB

Glycobiology

Polypeptide N-acetylgalactosaminyltransferase 18 Retains in Endoplasmic Reticulum Depending on its Luminal Regions Interacting with ER resident UGGT1, PLOD3 and LPCAT1.

Authors - Wenjuan Jia
mouseWB

Biochem Biophys Res Commun

UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner.

Authors - Jaeyong Cho
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