验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, mouse large intestine tissue, mouse kidney tissue, mouse spleen tissue, L02 cells, mouse liver tissue, rat liver tissue, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human normal colon, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy UQCRC1 Ready-To-Use IHC Kit. UQCRC1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21705-1-AP targets UQCRC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | UQCRC1 fusion protein Ag16322 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquinol-cytochrome c reductase core protein I |
| 别名 | Ubiquinol-cytochrome-c reductase complex core protein 1, QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I |
| 计算分子量 | 480 aa, 53 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC009586 |
| 基因名称 | UQCRC1 |
| Gene ID (NCBI) | 7384 |
| RRID | AB_10734437 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31930 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
| IHC protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
| IF protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
| IP protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Bull (Beijing) Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects | ||
Biomaterials Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. | ||
Theranostics Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer. | ||
Acta Pharmacol Sin MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by targeting SIRT3 to provoke mitochondrial malfunction. | ||
Int J Nanomedicine Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect | ||
Aging (Albany NY) Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism. |
