• Featured Product
  • KD/KO验证

USP36 Polyclonal antibody

USP36 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 14783-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, IP, ELISA and More (3)

Ubiquitin thioesterase 36, Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14783-1-AP targets USP36 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 USP36 fusion protein Ag6411 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 36
别名 Ubiquitin thioesterase 36, Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1
计算分子量 1123 aa, 123 kDa
观测分子量 100-150 kDa
GenBank蛋白编号BC038983
基因名称 USP36
Gene ID (NCBI) 57602
RRIDAB_2213357
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P275
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for USP36 antibody 14783-1-APDownload protocol
IF protocol for USP36 antibody 14783-1-APDownload protocol
IP protocol for USP36 antibody 14783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Med

Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis.

Authors - Luigina Romani
human,mouseWB,IF

Nat Commun

USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress

Authors - Kewei Qin
  • KD Validated
humanWB,IF,IP

Nucleic Acids Res

The deubiquitinase USP36 Regulates DNA replication stress and confers therapeutic resistance through PrimPol stabilization.

Authors - Yuanliang Yan
  • KD Validated
humanWB

Cell Death Differ

Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.

Authors - Yunhua Peng
  • KO Validated
mouseWB

Int J Mol Sci

Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity.

Authors - Marilena Pariano
humanWB, IHC, IF

Front Cell Dev Biol

USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway.

Authors - Suwei Zhu
Loading...