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VDAC1/2 Polyclonal antibody
VDAC1/2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (2)
Conjugate
Unconjugated
165
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse kidney tissue, 4T1 cells, Jurkat cells, C6 cells, rat kidney tissue, MCF-7 cells, MDA-MB-453s cells |
Positive IHC detected in | human colon tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver cancer tissue, mouse liver tissue |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:100-1:400 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10866-1-AP targets VDAC1/2 in WB, IHC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, rabbit, monkey, zebrafish, pieris rapae |
Immunogen | VDAC1/2 fusion protein Ag1144 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | voltage-dependent anion channel 1 |
Synonyms | Porin, VDAC1, VDAC1/Porin, Porin 31HM, Porin 31HL |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC008482 |
Gene Symbol | Porin |
Gene ID (NCBI) | 7416 |
RRID | AB_2257153 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P21796 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for VDAC1/2 antibody 10866-1-AP | Download protocol |
IHC protocol for VDAC1/2 antibody 10866-1-AP | Download protocol |
IF protocol for VDAC1/2 antibody 10866-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Nat Cell Biol AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1. | ||
Cell Rep Med Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults. | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. | ||