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VEGFA Polyclonal antibody

VEGFA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

IHC, WB, ELISA and More (4)

Conjugate

Unconjugated

Cat No : 19003-1-AP

Print datasheet

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF



经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

19003-1-AP targets VEGFA in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat, monkey, pig, rabbit, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFA fusion protein Ag13500
Full Name vascular endothelial growth factor A
Synonyms L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight45 kDa, 23 kDa
GenBank accession numberBC065522
Gene symbol VEGFA
UniprotP15692
Gene ID (NCBI) 7422
RRIDAB_2212657
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

实验方案

Product Specific Protocols
WB protocol for VEGFA antibody 19003-1-APDownload protocol
IHC protocol for VEGFA antibody 19003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bone

Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis.

Authors - Jiadong Wu
mouseWB

Dose Response

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

Authors - Xin Jiang
humanWB

J Biomed Nanotechnol

Nano-Gold Loaded with Resveratrol Enhance the Anti-Hepatoma Effect of Resveratrol In Vitro and In Vivo.

Authors - Duoduo Zhang
humanWB

Sci Rep

Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.

Authors - Zhaoxing Li
ratIHC

Stem Cell Res Ther

Human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation improves ovarian function in rats with premature ovarian insufficiency (POI) at least partly through a paracrine mechanism.

Authors - Li Ling
ratWB

Transl Neurosci

Fangchinoline Ameliorates the Expressions of Angiogenic Molecule in Cerebral Ischemia Induced Neuronal Degeneration in Neonatal Rats.

Authors - Han Daicheng